Candace Cham, PhDResearch Professional
My main projects deal with studying gene expression in the GI tract. First, I am interested in differential gene expression among spatial regions of the colon. To this end, I am using laser microdissection to isolate specific regions of the mouse colon and analyzing patterns of gene expression by qPCR and microarray. Second, I am growing epithelial cells isolated from mouse small intestine- or colon-derived crypt stem cells to study various aspects of epithelial cell function. We have also begun to grow human epithelial cells isolated from endoscopal biopsies in a similar fashion. Lastly, I am working with Vanessa Leone and Kristina Martinez to study in vitro culture models of liver cells to determine how diet and gut metabolites influence liver circadian clock and liver cell function.